Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5888807

Title:Devices for maintaining and growing human stem and/or hematopoietics cells
Inventors:Palsson, Bernhard O.; Ann Arbor, MI, USA
Emerson, Stephen G.; Ann Arbor, MI, USA
Schwartz, Richard M.; Ann Arbor, MI, USA
Summary:Described herein is a bioreactor and associated compositions for the culture of human stem and hematopoietic cells, comprised of a culture chamber through which a culture medium and respiratory cells are circulated. Further disclosed are methods by which various cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors that are continuously maintained in the bioreactor while undesirable metabolic products are removed. Claims of the invention include the ex vivo reconstruction of hematopoietic tissue from multiple cell types simultaneously cultured according to this process. Growth factors that are excreted by transfected stromal cells such as heterologous cells are also disclosed, as are techniques for the separate maintenance of stromal and hematopoietic cells, and for the harvesting of both the adherent and non-adherent cells.
Abstract:Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The invention also allows for the separate maintenance of stromal and hematopoietic cells, and to allow for harvesting of both the adherent and non-adherent cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5888807
Application Number:US1995000486247
Date Filed:07/06/1995
Date Published:30/03/1999
Assignee:The Regents of the University of Michigan, Ann Arbor, MI, USA


 
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