Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5906724

Title:Apparatus for separation of nucleated blood cells from a blood sample
Inventors:Sammons, David W.; Tucson, AZ, USA
Twitty, Garland E.; Tucson, AZ, USA
Utermohlen, Joseph G.; Tucson, AZ, USA
Sharnez, Rizwan; Sicklerville, NJ, USA
Summary:Described herein is an apparatus for the recovery of rare nucleated cells, especially fetal cells, using a new gravity-biased, counterflow stabilized electrophoretic separator. This invention introduces a charge-flow separation (CFS) device for the enrichment of such cells, such that the device employs a separation mechanism by which the rare cell fractions are recovered from whole fractions according to their relative-charge density and/or relative binding affinity for a leukocyte depletion solid phase matrix. The apparatus is designed with an internal cooling system which allows for the dissipation of heat generated by the electric field of the apparatus. The cooling system is described as consisting of a plurality of cooling tubes to circulate the coolant material, thereby preventing the cellular degradation that typically results from the high temperatures generated by the electric field. The use of a higher voltage gradient to shorten separation times is thus possible. Further provided are therapeutic applications such as in prenatal screening for genetic diseases.
Abstract:A charge-flow separation apparatus (CFS) for enriching rare cell populations, particularly fetal cells, from a whole blood sample by separating the rare cell fractions from whole fractions according to the relative-charge density and/or the relative binding affinity for a leukocyte depletion solid phase matrix is described. The apparatus having an internal cooling system allows for dissipating heat generated by the electric field of the apparatus. The internal cooling system, consisting of a plurality of cooling tubes to circulate coolant material, prevents cellular degradation typically associated with the high heat generated by the electric field and permits the use of a higher voltage gradient to shorten separation times.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5906724
Application Number:US1997000899283
Date Filed:23/07/1997
Date Published:25/05/1999
Assignee:BioSeparations, Inc., Tucson, AZ, USA


 
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