Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5908782

Title:Chemically defined medium for human mesenchymal stem cells
Inventors:Marshak, Daniel R.; Cold Spring Harbor, NY, USA
Holecek, James J.; Solon, OH, USA
Summary:Described herein are methods and materials for the expansion and maintenance of human mesenchymal precursor cells in a serum-free environment. Compositions include a minimum essential medium, serum albumin, an iron source, insulin or an insulin-like growth factor, glutamine, a mitogen selected from the group consisting of platelet derived growth factor and a serotonergic agonist which are present in amounts effective to culturally expand the human mesenchymal stem cells. Experimental applications in tissue culture media are provided.
Abstract:A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes (1) a minimum essential medium; (2) serum albumin; (3) an iron source; (4) insulin or an insulin-like growth factor; and (5) at least one amino acid selected from the group consisting of glutamine, arginine and and cysteine, and is free of serum. Also, a composition and method for culture expanding human mesenchymal precursor cells in a serum-free environment. This composition further includes a mitogen, paricularly a serotonergic agonist. The cells are preferably isolated human mesenchymal stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5908782
Application Number:US1995000464599
Date Filed:05/06/1995
Date Published:01/06/1999
Assignee:Osiris Therapeutics, Inc., Baltimore, MD, USA


 
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