Methods comprising apoptosis inhibitors for the generation of transgenic pigs |
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| Title: | Continuous process for the separation of biologic components from heterogeneous cell populations |
| Inventors: | Lake, William C.; Laguna Niguel, CA, USA Giesler, Richard; Deerfield, IL, USA Van Epps, Dennis; Cary, IL, USA Chapman, John R.; Lake Villa, IL, USA Martinson, Jeffrey A.; Mundelein, IL, USA Ellis, Dale R.; Wonder Lake, IL, USA Aono, Frederick; Arlington Heights, IL, USA Bischof, Daniel F.; McHenry, IL, USA |
| Summary: | Described herein are methods of selectively separating specific cell population from a heterogeneous cell mixture. The invention relates to a process by which the techniques of immunoaffinity separation and continuous flow centrifugal separation are combined in order to yield the desired separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. Methods of separation and rapid processing of large volumes of the cell mixture with a high degree of accuracy are disclosed. In a preferred embodiment of the invention, hematopoietic cells are selected from a group consisting of all leukocyte subpopulations and pluripotent stem cells. |
| Abstract: | A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US5916743 |
| Application Number: | US1995000467818 |
| Date Filed: | 06/06/1995 |
| Date Published: | 29/06/1999 |
| Assignee: | Baxter International Inc., Deerfield, IL, USA |