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Stem Cell Related Patent Number US5922601
| Title: | High efficiency gene trap selection of regulated genetic loci | | Inventors: | Baetscher, Manfred; Winchester, MA, USA
Nir, Waan-Jeng; Reading, MA, USA | | Summary: | This invention describes a novel gene trap useful for the identification of genes regulated in response to physiological and pathological processes. Fully detailed is a gene trap construct for identification of genes whose activity is regulated upon a cellular transition event which comprises in downstream sequence involving a cassette with a functional splice acceptor, a translation stop sequence and an internal ribosome entry site, and a promoterless protein coding sequence encoding at least one polypeptide providing positive and negative selection traits. Further disclosed is a method for identification of genes whose activity is regulated upon a cellular transition event by introducing the gene trap construct into a cell and observing expression of the positive and/or negative selection traits before and after the transition event. | | Abstract: | A gene trap construct for identification of genes whose activity is regulated upon a cellular transition event which comprises in downstream sequence (i) a cassette having a functional splice acceptor, a translation stop sequence and an internal ribosome entry site and (ii) a promoterless protein coding sequence encoding at least one polypeptide providing positive and negative selection traits. A method for identification of genes whose activity is regulated upon a cellular transition event by introducing the gene trap construct into a cell and observing expression of the positive and/or negative selection traits before and after the transition event. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5922601 | | Application Number: | US1996000716854 | | Date Filed: | 16/09/1996 | | Date Published: | 13/07/1999 | | Assignee: | BioTransplant, Inc., Charlestown, MA, USA |
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