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Stem Cell Related Patent Number US5928947
| Title: | Mammalian multipotent neural stem cells | | Inventors: | Anderson, David J.; Altadena, CA, USA
Stemple, Derek L.; Newton, MA, USA | | Summary: | Described herein are mammalian multipotent neural stem cells (NSCs) and novel methods for their production. The invention relates to the isolation, differentiation and properties of such NSCs and their progeny which, at the clonal level, are described as being capable of self-regeneration and asymmetrical division, while lineage restriction is demonstrated within developing clones which are sensitive to the local environment. Further disclosed are methods of transfecting such cells with foreign nucleic acid in order to produce an immortalized neural stem cell. Transplantation assays are provided for the identification of mammalian NSCs, as are methods for detecting antibodies to neural cell surface markers, including the monoclonal antibody to mouse LNGFR. | | Abstract: | The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5928947 | | Application Number: | US1995000483142 | | Date Filed: | 07/06/1995 | | Date Published: | 27/07/1999 | | Assignee: | California Institute of Technology, Pasadena, CA, USA |
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