Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5939598

Title:Method of making transgenic mice lacking endogenous heavy chains
Inventors:Kucherlapati, Raju; Darien, CT, USA
Jakobovits, Aya; Menlo Park, CA, USA
Summary:This invention introduces a method for producing transgenic mice lacking the expression of an endogenous immunoglobulin heavy chain. Described herein are the production of xenogeneic antibodies via such transgenic mice with inactivated endogenous immunoglobulin loci and functional human immunoglobulin loci. Further disclosed are methods by which such mice are produced according to repetitive transformations of embryonic stem cells by homologous recombination in conjunction with breeding, such that J segment genes are deleted from at least one endogenous heavy chain locus in the mouse embryonic stem cell by a targeting vector to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus. Therapeutic applications of such xenogeneic antibodies in the treatment of human disease are included.
Abstract:The subject invention provides non-human mammalian hosts characterized by inactivated endogenous Ig loci. The hosts are produced by repetitive transformations of embryonic stem cells by homologous recombination, preferably in conjunction with breeding. Different strategies are employed for recombination of the human loci randomly or at analogous host loci.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5939598
Application Number:US1992000922649
Date Filed:30/07/1992
Date Published:17/08/1999
Assignee:Abgenix, Inc., Fremont, CA, USA


 
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