Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5945577

Title:Cloning using donor nuclei from proliferating somatic cells
Inventors:Stice, Steven L.; Belchertown, MA, USA
Summary:Described herein is a novel method of cloning animals. In standard cloning, the process of somatic cell nuclear transfer (SCNT) involves the insertion of an adult donor cell into an enucleated oocyte, from which the cloned embryo is allowed to develop. This invention differs from the standard SCNT method in that the donor cell that is inserted into the enucleated oocyte is a proliferating somatic cell that has been expanded in culture, or it is a nucleus that has been isolated from such a somatic cell. From this process of nuclear transfer, a nuclear transfer (NT) unit is formed, which is then implanted into a surrogate host where the NT is allowed to develop. Claims of the invention include the ability to genetically modify the differentiated cell source of the donor nuclei, resulting in improved reliability in the multiplication of genotypes and transgenic genotypes, and in the efficiency of production of isogenic CICM cells including human isogenic embryonic or stem cells, and in the efficiency of production of genetically engineered or transgenic mammalian embryos, fetuses and offspring.
Abstract:An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5945577
Application Number:US1997000781752
Date Filed:10/01/1997
Date Published:31/08/1999
Assignee:University of Massachusetts as represented by its Amherst Campus, Amherst, MA, USA


 
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