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Stem Cell Related Patent Number US5948278
| Title: | System and method for enrichment of rare cell population from whole blood samples | | Inventors: | Sammons, David W.; Tucson, AZ, USA
Manley, Michael; Tucson, AZ, USA
Utermohlen, Joseph G.; Tucson, AZ, USA
Twitty, Garland E.; Tucson, AZ, USA | | Summary: | Described herein is a novel system for the enrichment of cell populations, especially fetal cells, from whole blood. The invention relates to the depletion of unwanted cells via methods of density gradient separation in a charged-flow separator. The rare cell fractions are separated from a whole blood sample according to the relative charge density and/or the relative binding affinity for a leukocyte depletion solid phase matrix. Methods are further provided by which the process of enrichment may be operated as a stand-alone process, or as a pre- or post-processing step in conjunction with the charge-flow separation method. Also disclosed are methods by which the cell population is selected from a group consisting of nucleated cells, erythroid progenitor cells and fetal nucleated erythrocytes within the whole blood sample. | | Abstract: | A method for enriching rare cell populations from a whole blood sample by separating rare cell fractions from whole blood sample according to the relative charge density and/or the relative binding affinity for a leukocyte depletion solid phase matrix is described. The enrichment method may be operated stand alone, or as a pre or post-processing step in conjunction with a charge-flow separation method. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5948278 | | Application Number: | US1997000949240 | | Date Filed: | 10/10/1997 | | Date Published: | 07/09/1999 | | Assignee: | BioSeparations, Inc., Tucson, AZ, USA |
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