Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5962325

Title:Three-dimensional stromal tissue cultures
Inventors:Naughton, Gail K.; La Jolla, CA, USA
Summary:This invention introduces living in vitro stromal tissue cultures, methods and uses thereof. Described herein are methods of culturing stromal cells that have been inoculated onto a three-dimensional framework in the presence of TGF-beta, such that the stromal cells include chondrocytes, chondrocyte-progenitors, fibroblasts, fibroblast-like cells, umbilical cord cells or bone marrow cells from umbilical cord blood. Methods are provided by which the cells are inoculated and grown on a three-dimensional framework optionally with the addition of cells derived from loose connective tissue such as endothelial cells, macrophages or monocytes, adipocytes, pericytes, and reticular cells found in bone marrow stroma. Claims of the invention include the maturation and segregation of the three-dimensional cells into components of adult tissue having interstitial spaces bridged by the mesenchymal stem cells. Therapeutic applications are included.
Abstract:The present invention relates to a method of stimulating the proliferation and appropriate cell maturation of a variety of different cells and tissues in three-dimensional cultures in vitro using TGF-.beta. in the culture medium. In accordance with the invention, stromal cells, including, but not limited to, chondrocytes, chondrocyte-progenitors, fibroblasts, fibroblast-like cells, umbilical cord cells or bone marrow cells from umbilical cord blood are inoculated and grown on a three-dimensional framework in the presence of TGF-.beta.. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal cells and connective tissue proteins naturally secreted by the stromal cells attach to and substantially envelope the framework composed of a biocompatible non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells. The living stromal tissue so formed provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts in vivo.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5962325
Application Number:US1998000157306
Date Filed:18/09/1998
Date Published:05/10/1999
Assignee:Advanced Tissue Sciences, Inc., La Jolla, CA, USA


 
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