Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US5977181

Title:Therapeutic uses for nitric oxide inhibitors
Inventors:Enikolopov, Grigori N.; Cold Spring Harbor, NY, USA
Peunova, Natalia I.; Cold Spring Harbor, NY, USA
Kuzin, Boris A.; Moscow, Russian Federation
Michurina, Tatiyana; Moscow, Russian Federation
Summary:Described herein are therapeutic uses of inhibitors of nitric oxide (NO) or nitric oxide synthase (NOS). Central to the invention is the discovery that NO is an important growth regulator in an intact developing organism, and these inventors propose methods of increasing in vivo a mammalian population of hematopoietic stem cells in bone marrow via the use of an inhibitor of NO and NOS. Claims of the invention include the production of bone marrow with an increased population of hematopoietic stem cells capable of undergoing normal hematopoiesis and differentiation after the bone marrow has been contacted with such inhibitors. Further disclosed are in vivo methods of increasing a mammalian population of cells in the S phase via contacting the tissue with an inhibitor of NO or NOS. Therapeutic applications are included for the regeneration of selected mammalian tissue.
Abstract:The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation, wherein the bone marrow is contacted with an inhibitor of NO, such as an inhibitor of nitric oxide synthase (NOS), thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with an inhibitor of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.
US Patent Website:Click Here for Full Text of Patent
Title Number:US5977181
Application Number:US1997000969475
Date Filed:13/11/1997
Date Published:02/11/1999
Assignee:Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA


 
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