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Stem Cell Related Patent Number US5994088
| Title: | Methods and reagents for preparing and using immunological agents specific for P-glycoprotein | | Inventors: | Mechetner, Eugene; Irvine, CA, USA
Roninson, Igor B.; Wilmette, IL, USA | | Summary: | Described herein is an immunological reagent specific for P-glyco:protein, methods and uses thereof. The invention relates to immunological reagents and methods specific for a mammalian transmembrane protein, Pgp, having a non-specific efflux pump activity which has been identified as a component of multidrug resistance in cancer patients undergoing chemotherapy. Further disclosed are methods for developing and using immunological reagents specific for certain mutant forms of Pgp and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP-depleting agents. Claims of the invention include improved methods for purifying hematopoietic stems cells expressing Pgp. Therapeutic applications include diagnostic evaluations of cancer cells expressing Pgp, as well as uses in the detection of multi-drug resistant cancer, in the isolation of hematopoietic cells and in the selective killing of specific cells. | | Abstract: | This invention relates to immunological reagents and methods specific for a mammalian, transmembrane protein termed Pgp, having a non-specific efflux pump activity established in the art as being a component of clinically-important multidrug resistance in cancer patients undergoing chemotherapy. The invention provides methods for developing and using immunological reagents specific for certain mutant forms of Pgp and for wild-type Pgp in a conformation associated with substrate binding or in the presence of ATP depleting agents. The invention also provides improved methods for purifying hematopoietic stems cells expressing Pgp and diagnostic and therapeutic methods for cancer cells expressing Pgp. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US5994088 | | Application Number: | US1996000752447 | | Date Filed: | 15/11/1996 | | Date Published: | 30/11/1999 | | Assignee: | Board of Trustees of the University of Illinois, Urbana, IL, USA |
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