Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6007993

Title:In vitro test for embryotoxic and teratogenic agents using differentiation-dependent reporter expression in pluripotent rodent embryonic cells
Inventors:Wobus, Anna Magdalene; Gatersleben, Germany
Summary:Described herein are in vitro methods of detecting chemically-induced embryo-toxic and teratogenic effects of chemicals according to their effects on differentiated pluripotent embryonic stem (ES) cell or embryonic germ (EG) cell lines that have been obtained from the primordial germ cells of a mouse or rat carrying and which carry reporter gene under the control of a tissue-specific promoter. The invention relates to a test procedure characterized by stable transgenic ES or EG cell clones containing tissue-specific promoters and reporter genes, such that the differentiation-dependent expression of tissue-specific genes is carried out following the differentiation of ES cells in the presence of embryotoxic substances acting at specific times and resulting in different germination path derivatives. Further disclosed are the means by which chemically induced activation is detected such that tissue-specific genes which regulate embryonic development are repressed or modulated accordingly. Claimed advantages of the invention include the elimination of the necessity for animal testing.
Abstract:The invention concerns an in vitro test procedure for the detection of chemically induced effects on embryonic development and for differentiation for the purpose of embryotoxicity/teratogenicity screening based on differentiated pluripotent embryonic stem (ES) cells from mice and rats using embryonic germ (EG) cells obtained from primoridial germ cells. The proposed test procedure is characterised in that stable transgenic ES or EG cell clones containing tissue-specific promoters and reporter genes are selected, differentiation-dependent expression of tissue-specific genes is carried out following differentiation of ES cells in the presence of embryotoxic substances acting at specific times into different germination path derivatives; this is followed by detection of chemically induced activation, repression or modulation of tissue-specific genes which regulate embryonic development.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6007993
Application Number:US1998000983147
Date Filed:24/02/1998
Date Published:28/12/1999
Assignee:Insitut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany


 
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