Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6018096

Title:Animal model for engraftment, proliferation and differentiation of human hematopoietic stem cells
Inventors:Keating, Armand; Toronto, Canada
Wu, Dong-dong; Toronto, Canada
Summary:This invention introduces an animal model for the production of human tissue. Disclosed herein is a chimeric nonhuman genetically-immunocompetent mammal with a hematopoietic system consisting of cells of human origin and some lymphoid hematopoietic cells syngeneic to the mammal itself. The chimeric nonhuman genetically-immunocompetent mammal is selected from the group consisting of a mouse, a rat, a rabbit, a cat, a dog, a pig, a sheep and a non-human primate, wherein the hematopoietic system of the mammal consists of human passaged bone marrow stromal cells and human hematopoietic stem cells obtained from a CD34+ -enriched bone marrow fraction, such that the mammal contains transplanted syngeneic non-lymphoid spleen colony cells. Claims of the invention include human hematopoietic cells which may be obtained from the chimeric, non-human mammal. Therapeutic applications are included for the engraftment, proliferation and differentiation of human hematopoietic cells derived according to this model.
Abstract:A chimeric, non-human, genetically-immunocompetent mammal is disclosed. The hematopoietic system of the mammal has human passaged bone marrow stromal cells and human hematopoietic stem cells obtained from a CD34.sup.+ -enriched bone marrow fraction, and also contains transplanted syngeneic non-lymphoid spleen colony cells. The mammal may be a mouse, a rat, a rabbit, a cat, a dog, a pig, a sheep or a non-human primate. The mammal can be produced by providing a non-human, genetically-immunocompetent mammal in which its immunologic genotype comports with the norm for the species of the mammal, exposing the mammal to a level of x- or gamma-radiation that is sufficient to destroy substantially all bone marrow in the mammal to render the mammal phenotypically immunodeficient, then transplanting into the mammal syngeneic non-lymphoid spleen colony cells and human passaged bone marrow stromal cells, and transplanting into the mammal human hematopoietic stem cells obtained from a CD34.sup.+ -enriched bone marrow fraction. Human hematopoietic cells may be obtained from the chimeric, non-human mammal.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6018096
Application Number:US1993000055105
Date Filed:03/05/1993
Date Published:25/01/2000
Assignee:Surrogen, Inc., Vancouver


 
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