Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6033907

Title:Enhanced virus-mediated DNA transfer
Inventors:Williams, David A.; Indianapolis, IN, USA
Summary:Described herein are methods of enhanced virus-mediated DNA transfer via the incorporation of fibronectin or fragments thereof. The invention relates to a process for increasing the efficiency of transduction of hematopoietic and other cells, thereby yielding a transduced population of viable mammalian cells, with the use of retroviruses according to a method of infecting the cells in the presence of fibronectin or fibronectin fragments that exhibit virus-binding and cell-binding domains. Claims of the invention include enhanced retroviral-mediated gene transfer by the fibronectin and fibronectin fragments into the cells, particularly the hematopoietic cells including committed progenitors and primitive hematopoietic stem cells, and improved methods for somatic gene therapy which capitalize on enhanced gene transfer. Therapeutic applications include increased transduction efficiency in gene therapy.
Abstract:A method to increase the efficiency of transduction of hematopoietic and other cells by retroviruses includes infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. The invention also provides improved methods for somatic gene therapy capitalizing on enhanced gene transfer, hematopoietic cellular populations, and novel constructs for enhancing retroviral-mediated DNA transfer into cells and their use.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6033907
Application Number:US1995000536891
Date Filed:29/09/1995
Date Published:07/03/2000
Assignee:Indiana University Foundation, Bloomington, IN, USA


 
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