Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6051427

Title:Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
Inventors:Finer, Mitchell H.; San Carlos, CA, USA
Roberts, Margo R.; Charlottesville, VA, USA
Dull, Thomas L.; San Francisco, CA, USA
Zsebo, Krisztina M.; Cupertino, CA, USA
Qin, Lu; Lutherville, MD, USA
Farson, Deborah A.; Oakland, CA, USA
Summary:Described herein is a high efficiency retroviral packaging plasmid system for the production of high titers. The invention relates to a novel retroviral packaging system in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells, after which high titers of recombinant retrovirus are subsequently produced in the transfected mammalian cells and can be used to transduce a mammalian target cell by cocultivation or supernatant infection. Claims of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. Therapeutic applications are included for the transduction of human cells such as hematopoietic stem cells, T or B cells with foreign genes.
Abstract:The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6051427
Application Number:US1995000517488
Date Filed:21/08/1995
Date Published:18/04/2000
Assignee:Cell Genesys, Inc., Foster City, CA, USA


 
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