Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6060270

Title:Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
Inventors:Humes, H. David; Ann Arbor, MI, USA
Summary:Described herein are ex vivo renal tubule stem cells, methods and uses thereof. The invention relates to a tubule system prepared by a process of culturing a medium which contains an all-trans retinoic acid, transforming growth factor-?1 and either epidermal growth factor or transforming growth factor-?a in amounts effective for achieving tubulogenesis, which is defined herein as a phenotypic transformation of cells such that condensed aggregates of tubule cells form about a central lumen which is bordered by cells possessing a polarized epithelial phenotype with extensive microvilli formation and tight junctional complexes along the lumenal border. Genetic applications in renal therapy are provided.
Abstract:Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-.beta..sub.1, epidermal growth factor, and all-trans retinoic acid.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6060270
Application Number:US1995000449912
Date Filed:25/05/1995
Date Published:09/05/2000
Assignee:The University of Michigan, Ann Arbor, MI, USA


 
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