Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6077987

Title:Genetic engineering of cells to enhance healing and tissue regeneration
Inventors:Breitbart, Arnold S.; Great Neck, NY, USA
Grande, Daniel S.; Sea Cliff, NY, USA
Mason, James M.; Bethpage, NY, USA
Summary:Described herein are methods of modifying wound healing, cell proliferation or differentiation. The invention relates to a process of enhancing and increasing the efficiency of the repair of tissue, primarily bone or cartilage, using genetically engineered cells such as mesenchymal stem cells which have been isolated from periosteum tissue and transfected with a gene encoding a growth factor for the particular cell type to be repaired. Specific therapeutic examples are provided.
Abstract:A method for enhancing and/or increasing the efficiency of repair of tissues, primarily bone or cartilage, using genetically engineered cells has been developed. In the preferred embodiment, mesenchymal stem cells are isolated from periosteum tissue, and transfected with the gene encoding a growth factor for the particular cell type to be repaired. For example, for repair of bone, a gene (or genes) encoding bone morphogenic protein is transfected into periosteal cells. The transfected periosteal cells then express the bone morphogenic protein in culture to promote bone repair as a function of the expressed bone morphogenic protein. Cells can be transfected using any appropriate means, including viral vectors, as shown by the example, chemical transfectants, or physico-mechanical methods such as electroporation and direct diffusion of DNA. Genes can encode any useful protein, for example, a specific growth factor, morphogenesis factor, a structural protein, or a cytokine which enhances the temporal sequence of wound repair, alters the rate of proliferation, increases the metabolic synthesis of extracellular matrix proteins, or directs phenotypic expression in endogenous cell populations. Representative genes encoding proteins include bone growth factor genes, cartilage growth factor genes, nerve growth factor genes, and general growth factors important in wound healing, such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), epidermal growth factor (EGF), basic fibroblast growth factor (FGF), endothelial derived growth supplement.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6077987
Application Number:US1997000923718
Date Filed:04/09/1997
Date Published:20/06/2000
Assignee:North Shore-Long Island Jewish Research Institute, Manhasset, NY, USA


 
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