Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6096540

Title:Differentiation of granulocytic and monocytic progenitor cells
Inventors:Olweus, Johanna; Palo Alto, CA, USA
Lund-Johansen, Fridtjof; Palo Alto, CA, USA
Thompson, Peter; Danville, CA, USA
Summary:Described herein are methods of distinguishing monocytic and granulocytic pre-cursors from CD34-positive cells via a process of measuring the level of expression of macrophage-colony stimulating factor receptor and isolated precursor cells. The invention relates to a method of distinguishing progenitors committed to the granulo-monocytic lineage in a population containing CD34+ cells, by contacting the cells with antibodies specific for CD34, M-CSFR and CD64, which is followed by a process of detection via flow cytometry by which the cells that are CD34hi M-CSFR+ CD64- are identified and from which a CD34hi M-CSFR+ CD64- phenotype indicates granulo-monocytic lineage progenitor cells.
Abstract:
US Patent Website:Click Here for Full Text of Patent
Title Number:US6096540
Application Number:US1996000724725
Date Filed:30/09/1996
Date Published:01/08/2000
Assignee:Becton Dickinson and Company, Franklin Lakes, NJ, USA


 
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