Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6114598

Title:Generation of xenogeneic antibodies
Inventors:Kucherlapati, Raju; Darien, CT, USA
Jakobovits, Aya; Menlo Park, CA, USA
Kalpholz, Sue; Stanford, CA, USA
Brenner, Daniel G.; Redwood City, CA, USA
Capon, Daniel J.; Hillsborough, CA, USA
Summary:Described herein is a genome of embryonic stem cells which has been modified by yeast spheroplasts containing a yeast artificial chromosome comprised of a xenogeneic DNA segment. The invention relates to non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human Ig loci for response to an immunogen which produces human antibodies or analogs thereof. Products of the invention include chimeric and transgenic mice which exhibit stably integrated large, xenogeneic DNA segments which have been introduced by fusion with yeast spheroplasts comprised of yeast artificial chromosomes (YACs) and which include the xenogeneic DNA segments as well as a selective marker such as HPRT, and embryonic stem cells. Therapeutic applications are included for the testing of toxicity and drug development, in the development of derivative cell lines and in the isolation of genes or proteins involved in cellular differentiation. This invention represents a continuation of other applications filed by the same assignee and inventors.
Abstract:The subject invention provides non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human Ig loci for response to an immunogen to produce human antibodies or analogs thereof. The hosts are produced by multiple genetic modifications of embryonic cells in conjunction with breeding. Different strategies are employed for recombination of the human loci randomly or at analogous host loci. Chimeric and transgenic mammals, particularly mice, are provided, having stably integrated large, xenogeneic DNA segments. The segments are introduced by fusion with yeast spheroplasts comprising yeast artificial chromosomes (YACs) which include the xenogeneic DNA segments and a selective marker such as HPRT, and embryonic stem cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6114598
Application Number:US1995000464582
Date Filed:05/06/1995
Date Published:05/09/2000
Assignee:Abgenix, Inc., Fremont, CA, USA


 
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