Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6139833

Title:Targeted gene discovery
Inventors:Lexicon Genetics Incorporated, The Woodlands, TX, USA
Summary:This invention describes a comprehensive system for targeted gene discovery employing the use of infectious viruses. Such viruses are composed of a recombinant viral genome and a chimeric integrase, thus affording a retrovirus that has been engineered to exhibit increased accessibility to genomic DNA. Alternately, the retrovirus may mutate and identify the chromosomal target sequences of DNA binding proteins. Employed is the combination of retroviral integrase/DNA binding protein fusion constructs and gene-trapping methodologies, which provide the ability to establish proviral integration at any location within the genome. Also described is the generation of a collection of eukaryotic cells in which each cell contains a mutation in a target gene or sequence for a known DNA binding protein which also allow for rapid in vivo functional analysis.
Abstract:The present invention describes a comprehensive system for gene discovery using retrovirus that have been engineered to exhibit increased accessibility to genomic DNA, or to mutate and identify the chromosomal target sequences of DNA binding proteins. The strategy employs the combination of retroviral integrase/DNA binding protein fusion constructs and gene-trapping methodologies. This novel technology provides the ability to establish proviral integration at any location within the genome. In addition, it allows for the generation of a collection of eukaryotic cells in which each cell contains a mutation in a target gene or sequence for a known DNA binding protein which also allow for rapid in vivo functional analysis. Sequence information obtained for genes identified using the described methods identify a collection of eukaryotic genes related by, or directly or indirectly regulated by, a given DNA binding protein.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6139833
Application Number:US1997000907598
Date Filed:08/08/1997
Date Published:31/10/2000
Assignee:Burgess, Rob; Houston, TX, USA Friedrich, Glenn; The Woodlands, TX, USA Zambrowicz, Brian; The Woodlands, TX, USA Sands, Arthur; The Woodlands, TX, USA


 
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