Methods comprising apoptosis inhibitors for the generation of transgenic pigs |
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| Title: | Homologous recombination for allogeneic donor cells |
| Inventors: | Kucherlapati, Raju; Darien, CT Koller, Beverly H.; Carrboro, NC Smithies, Oliver; Chapel Hill, NC Dubridge, Robert B.; Belmont, CA Greenburg, Gary; San Carlos, CA Capon, Daniel J.; Hillsborough, CA Williams, Steven R.; San Francisco, CA De Rafael, Mariona Lourdes Arbones; Barcelona, Spain |
| Summary: | This invention describes the use of homologous recombination to inactivate genes associated with antigenic expression, thereby yielding a cell line that may be used as allogeneic donor cells. The genetic engineering ex vivo of the mammalian cell line yields a diminished rejection response due to the lack of at least one functional Major Histocompatibility Complex (MHC) antigen. Methods for reducing and eliminating the expression of these Class 1 MHC antigens, as well as the precise methods by which homologous recombination is applied to the non-transformed mammalian somatic cells, are also described. |
| Abstract: | Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2- microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as allogeneic donor cells. Methods for homologous recombination in non-transformed mammalian somatic cells are also described. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6139835 |
| Application Number: | US1995000443647 |
| Date Filed: | 18/05/1995 |
| Date Published: | 31/10/2000 |
| Assignee: | Cell Genesys, Inc., Foster City, CA, USA |