Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6139835

Title:Homologous recombination for allogeneic donor cells
Inventors:Kucherlapati, Raju; Darien, CT
Koller, Beverly H.; Carrboro, NC
Smithies, Oliver; Chapel Hill, NC
Dubridge, Robert B.; Belmont, CA Greenburg, Gary; San Carlos, CA
Capon, Daniel J.; Hillsborough, CA
Williams, Steven R.; San Francisco, CA
De Rafael, Mariona Lourdes Arbones; Barcelona, Spain
Summary:This invention describes the use of homologous recombination to inactivate genes associated with antigenic expression, thereby yielding a cell line that may be used as allogeneic donor cells. The genetic engineering ex vivo of the mammalian cell line yields a diminished rejection response due to the lack of at least one functional Major Histocompatibility Complex (MHC) antigen. Methods for reducing and eliminating the expression of these Class 1 MHC antigens, as well as the precise methods by which homologous recombination is applied to the non-transformed mammalian somatic cells, are also described.
Abstract:Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, the .beta..sub.2- microglobulin gene is inactivated for reducing or eliminating the expression of functional Class I MHC antigens. The resulting cells may be used as allogeneic donor cells. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6139835
Application Number:US1995000443647
Date Filed:18/05/1995
Date Published:31/10/2000
Assignee:Cell Genesys, Inc., Foster City, CA, USA


 
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