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Stem Cell Related Patent Number US6140119
| Title: | Expression of estrogen receptors in type I and type II human breast epithelial cells | | Inventors: | Chang, Chia-Cheng; Okemos, MI, USA | | Summary: | This invention introduces novel purified type I human breast epithelial cells expressing a truncated human estrogen receptor and capable of forming budding or ductal structures when cultured in a matrix of reconstituted basement membrane components. Therapeutic applications in the treatment of anemia and leukemia are provided. | | Abstract: | A method is described for culturing morphologically and antigenically distinguishable types of normal human breast epithelial cells (HBEC) derived from reduction mammoplasty. Type I HBEC showed luminal and stem cell characteristics i.e. the ability to form budding/ductal structures when cultured on MATRIGEL and expressed a variant ER (.about.48 Kd) which has a deletion in the DNA binding domain (exon 2). In contrast, Type II HBEC with basal epithelial phenotype are ER-negative when cultured on MATRIGEL. Simian virus 40 (SV40) transformed Type I and Type II HBEC lines also expressed the variant ER. Tumors formed in athymic nude mice by in vitro transformed tumorigenic Type I cell lines, however, expressed a high level of wild type ER which was undetectable in these cells grown in vitro before and after tumor formation. Thus, there appears to be a differentional ER mRNA splicing between the in vitro and in vivo milieu. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6140119 | | Application Number: | US1997000790659 | | Date Filed: | 29/01/1997 | | Date Published: | 31/10/2000 | | Assignee: | Michigan State University, East Lansing, MI, USA |
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