Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6146888

Title:Method of enriching for mammalian stem cells
Inventors:Smith, Austin Gerard; Edinburgh, United Kingdom
Mountford, Peter Scott; Elsfernwid, Australia
Summary:Described herein are methods of isolation, enrichment and the selective propagation of mammalian stem cells. The invention relates to a process of transfecting the cells with a selectable marker such as an antibiotic resistance gene, the differential expression of which allows for the selection and maintenance of the cells. Further described are properties of the mammalian stem cells such that their genome contains at least one nucleic acid construct encoding an antibiotic resistance gene that is operatively linked to a promoter specific for the mammalian stem cells, such that the preferential expression of the antibiotic resistance gene in the stem cells results in the preferential survival of the stem cells in the presence of antibiotic.
Abstract:Mammalian stem cells are obtained and maintained in vitro whose genome has at least one nucleic acid construct encoding an antibiotic resistance gene operatively linked to a promoter specific for mammalian stem cells. The preferential expression of the antibiotic resistance gene in the stem cells results in the preferential survival of the stem cells in the presence of antibiotic.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6146888
Application Number:US1995000535141
Date Filed:29/12/1995
Date Published:14/11/2000
Assignee:The University of Edinburgh United Kingdom


 
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