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Stem Cell Related Patent Number US6194635
| Title: | Embryonic germ cells, method for making same, and using the cells to produce a chimeric porcine | | Inventors: | Anderson, Gary B.; Davis, CA, USA
Shim, Hosup; Davis, CA, USA | | Summary: | Described herein are methods of preparing ungulate embryonic stem-like cells via the culturing of primordial germ cells collected from an ungulate embryo. The invention relates to methods by which primordial germ cells are extracted from post blastocyst porcine embryos by extracting primordial germ cells from the gonadal ridges of 25-day-old porcine embryos. After culturing, the primordial germ cells yield to cells which resemble embryonic stem cells in morphology and pluripotency. In one advantageous embodiment of the invention the cells are maintainable for several months in culture and can be genetically manipulated using homologous recombination technology in order to insert desired genetic material into the genetic complement of the cell at a specific location. Also provided is a genetically manipulated cell which can be inserted into a porcine blastocyst to produce a chimeric porcine. This invention represents a continuation of other applications filed by the same assignee and inventors. | | Abstract: | Primordial germ cells are extracted from post blastocyst piorcine embryos such as extracting primordial germ cells from the gonadal ridges of 25-day porcine embryos. The primordial germ cells are cultured in long term culture (over 30 days) resulting in cells which resemble embryonic stem cells in morphology and with respect to maintaining pluripotency. The cells obtained can be maintained for several months in culture and can be genetically manipulated using homologous recombination technology in order to insert desired genetic material into the genetic complement of the cell at a desired location. The genetically manipulated cell can be inserted into a porcine blastocyst to produce a chimeric porcine. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6194635 | | Application Number: | US1996000717155 | | Date Filed: | 20/09/1996 | | Date Published: | 27/02/2001 | | Assignee: | The Regents of the University of California, Oakland, CA, USA |
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