Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6200806

Title:Primate embryonic stem cells
Inventors:Thomson, James A.; Madison, WI, USA
Summary:Described herein is a novel purified preparation of pluripotent primate embryonic stem cells, methods and uses thereof. The invention relates to a preparation which is characterized by the cell surface markers SSEA-1 (-), SSEA-4 (+), TRA-1-60 (+), TRA-1-81 (+), and alkaline phosphatase (+). Further disclosed are methods by which the cells of the preparation are human embryonic stem cells exhibiting normal karyotypes and which continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines are further described as retaining the ability to form trophoblasts and to differentiate into all tissues derived from all three embryonic germ layers, namely, the endoderm, mesoderm and ectoderm. Multiple embodiments of the invention are provided, as are therapeutic applications in the generation of transgenic non-human primates for models of genetic disease, and for tissue transplantation.
Abstract:A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (-); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6200806
Application Number:US1998000106390
Date Filed:26/06/1998
Date Published:13/03/2001
Assignee:Wisconsin Alumni Research Foundation, Madison, WI, USA


 
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