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Stem Cell Related Patent Number US6200812
| Title: | Sequence alterations using homologous recombination | | Inventors: | Pati, Sushma; Redwood City, CA, USA
Zarling, David A.; Menlo Park, CA, USA | | Summary: | This invention describes a method for targeting and altering, by homologous recombination, a pre-selected target nucleic acid sequence in an extrachromosomal sequence of a procaryotic cell. The modification of these target polynucleotide sequences by homologous recombination involves the use of recombinase and at least 2 single stranded polynucleotides complementary to each other, for the correction of such diseased alleles as those found in cystic fibrosis. Further disclosed are methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing. Also provided are methods for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. Such sequence alterations relate to the targeting of an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, thereby generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell. Such methods are generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention. | | Abstract: | The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, the invention relates to methods for targeting an exogenous polynucleotide having a linked chemical substituent to a predetermined endogenous DNA sequence in a metabolically active target cell, generating a DNA sequence-specific targeting of one or more chemical substituents in an intact nucleus of a metabolically active target cell, generally for purposes of altering a predetermined endogenous DNA sequence in the cell. The invention also relates to compositions that contain exogenous targeting polynucleotides, complementary pairs of exogenous targeting polynucleotides, chemical substituents of such polynucleotides, and recombinase proteins used in the methods of the invention. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6200812 | | Application Number: | US1999000288586 | | Date Filed: | 08/04/1999 | | Date Published: | 13/03/2001 | | Assignee: | SRI International, Menlo Park, CA, USA |
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