Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6207371

Title:Indexed library of cells containing genomic modifications and methods of making and utilizing the same
Inventors:Zambrowicz, Brian; The Woodlands, TX, USA
Friedrich, Glenn A.; The Woodlands, TX, USA
Bradley, Allan; Houston, TX, USA
Sands, Arthur T.; The Woodlands, TX, USA
Summary:This invention describes a library of genetically altered cells as a source of mutated cells for use in producing transgenic non-human animals, created by transfecting populations of cells with both DNA and viral vectors. Methods and vectors, both DNA and retroviral, are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
Abstract:Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6207371
Application Number:US1997000942806
Date Filed:02/10/1997
Date Published:27/03/2001
Assignee:Lexicon Genetics Incorporated, The Woodlands, TX, USA


 
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