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Stem Cell Related Patent Number US6225044
| Title: | Method for gene transfer into cells activated from a quiescent state | | Inventors: | Klein, Antoinette; Antony, France
Hatzfeld, Jacques; Antony, France | | Summary: | This invention introduces a method of determining the degree of maturity of stem cells according to a procedure for releasing stem cells from a quiescent state by blocking the cell cycle inhibitor. Described herein is a method for the transfer of a gene into a culture of primitive stem cells which comprises a prestimulation period that is time-limited to less than 36 hours in order to retain hematopoietic potential, and which involves the additional step of adding a blocking agent to block at least one inhibitor of a cell cycle of the primitive stem cells. Claims of the invention include a more efficient transfer and more stable expression, with therapeutic applications in the treatment of genetic diseases. | | Abstract: | A method is disclosed for gene transfer into a culture of primitive stem cells which comprises a prestimulation step of adding a blocking agent to block at least one inhibitor of a cell cycle of said primitive stem cells. The prestimulation is time-limited for a period of less than approximately 36 hours so that said culture of primitive stem cells retains hematopoietic potential. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6225044 | | Application Number: | US1999000287623 | | Date Filed: | 07/04/1999 | | Date Published: | 01/05/2001 | | Assignee: | Centre National de la Recherche Scientifique, Paris, France |
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