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Stem Cell Related Patent Number US6225119
| Title: | Production of megakaryocytes by the use of human mesenchymal stem cells | | Inventors: | Qasba, Pankaj; Columbia, MD, USA
Thiede, Mark A.; Forest Hill, MD, USA | | Summary: | Described herein are in vitro methods of production of megakaryocytes via the isolation of hematopoietic progenitor cells from a tissue specimen, such as marrow cells or peripheral blood, which are then co-cultured with human mesenchymal stem cells to induce megakaryocyte differentiation and platelet production. Further disclosed are methods by which the hematopoietic stem cells may be genetically engineered to carry genes of interest, particularly for the expression of physiologically active proteins. Therapeutic applications include the treatment of blood disorders and the acceleration of wound healing. | | Abstract: | The present invention is directed to hematopoietic progenitor cells isolated from a tissue specimen, such as marrow cells or peripheral blood, and to the method of co-culturing isolated hematopoietic progenitor cells with human mesenchymal stem cells to induce megakaryocyte differentiation and platelet production. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate blood disorders. | | US Patent Website: | Click Here for Full Text of Patent | | Title Number: | US6225119 | | Application Number: | US1999000316797 | | Date Filed: | 21/05/1999 | | Date Published: | 01/05/2001 | | Assignee: | Osiris Therapeutics, Inc., Baltimore, MD, USA |
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