Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6225122

Title:Human spinal cord cell lines and methods of use therefor
Inventors:Sah, Dinah W. Y.; San Diego, CA, USA
Li, Ronghao; San Diego, CA, USA
Summary:This invention describes methods of preparing conditionally immortalized human spinal cord neural precursor cells. Described herein are methods for transfecting human spinal cord cells plated on a first surface and in a first growth medium that permit proliferation with DNA encoding a selectable marker and an externally regulatable growth-promoting gene, which is followed by a process of selecting the transfected cells on a second surface and in a second growth medium which permit attachment and proliferation, and which thereby produce a conditionally-immortalized human spinal cord neural precursor cell. Also included are therapeutic uses in the prevention and treatment of a variety of spinal cord-related diseases and injuries, in assays for the general study of spinal cord cell development and differentiation, and for producing a conditionally-immortalized human spinal cord neural precursor cell. If such cell lines are clonal, they may also be used to generate neurons, including motor neurons.
Abstract:Conditionally-immortalized human spinal cord cell lines are provided. Such cell lines, which may be clonal, may be used to generate neurons, including motoneurons. The cell lines and/or differentiated cells may be used for the development of therapeutic agents to prevent and treat a variety of spinal cord-related diseases and injuries. The cell lines and/or differentiated cells may also be used in assays and for the general study of spinal cord cell development and differentiation.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6225122
Application Number:US1998000059790
Date Filed:13/04/1998
Date Published:01/05/2001
Assignee:Signal Pharmaceuticals, Inc., San Diego, CA, USA


 
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