Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6258998

Title:Method of cloning porcine animals
Inventors:Damiani, Philip; Madison, WI, USA
Betthauser, Jeffrey M.; Windsor, WI, USA
Forsberg, Erik J.; Fitchburg, WI, USA
Bishop, Michael D.; Rio, WI, USA
Summary:This invention describes materials and methods for cloning porcine animals. Detailed herein are totipotent cells useful for cloning porcine animals, porcine embryos produced from such cells by employing nuclear transfer techniques, and porcine animals that arise from such cells and such embryos. Fully disclosed are methods of obtaining a totipotent porcine nuclear donor cell from a cell culture, and forming a cybrid by translocating the totipotent porcine nuclear donor cell or nucleus into an enucleated porcine oocyte. Also included are methods by which, prior to enucleation, the oocyte has been matured for between 42 and 56 hours. Details are given for the culturing, translocation, maturation and activation of the oocyte, and for the development of the cybrid into a porcine embryo.
Abstract:The present invention relates to materials and methods for cloning porcine animals. The invention relates in part to totipotent cells useful for cloning porcine animals, porcine embryos produced from such cells by employing nuclear transfer techniques, and porcine animals that arise from such cells and embryos.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6258998
Application Number:US1998000199138
Date Filed:24/11/1998
Date Published:10/07/2001
Assignee:Infigen, Inc., DeForest, WI, USA


 
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