Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6271436

Title:Cells and methods for the generation of transgenic pigs
Inventors:Piedrahita, Jorge A.; College Station, TX
Bazer, Fuller W.; College Station, TX
Summary:This method of growing porcine primordial germ cells employs a novel cell culture in which the density of the feeder cells in the culture medium, in combination with a fibroblast growth factor, constitute a highly effective rate of differentiation. Specific methods of isolation and transformation of the primordial germ cells from the cultured cell lines into transgenic pigs are described.
Abstract:Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6271436
Application Number:US1997000949155
Date Filed:10/10/1991
Date Published:07/08/2001
Assignee:Texas A and M University System, College Station, TX, USA


 
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