Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6274378

Title:Methods and compositions for obtaining mature dendritic cells
Inventors:Steinman, Ralph M.; Westport, CT, USA
Bhardwaj, Nina; Montclair, NJ, USA
Schuler, Gerold; Spardorf, Germany
Summary:This invention introduces methods and materials for the in vitro production of mature dendritic cells from immature dendritic cells that have been derived from pluripotent cells. Described herein is a two-step improved process for the generation of CD83-positive mature dendritic cells from nonproliferating progenitors in human blood, via a priming phase followed by a differentiation phase. Further disclosed are methods by which a culture of T cell depleted mononuclear cells in a medium are supplemented with GM-CSF and IL-4 to produce immature dendritic cells, after which they are exposed to a monocyte conditioned medium and to IFN?a, a dendritic cell maturation factor. Claims of the invention include dendritic cells which possess all the features of mature cells including a stellate cell shape, nonadherence to plastic, and strong T cell stimulatory activity. Therapeutic applications include the activation of T cells against specific antigens in the treatment of various diseases.
Abstract:We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or priming phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or differentiation phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6274378
Application Number:US1998000179504
Date Filed:27/10/1998
Date Published:14/08/2001
Assignee:The Rockefeller University, New York, NY, USA


 
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