Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6303327

Title:Gene trap construct for identification and isolation of genes
Inventors:Von Melchner, Harald; D-60596 Frankfurt, Germany
Holzer, Dieter; D-60596 Frankfurt, Germany
Summary:Described herein is a gene trap construct comprised of a first reporter gene that activates a second reporter gene, which is useful for the identification and isolation of genes, especially transiently expressed genes. Further described is a cell, preferably a mammalian cell, containing such a gene-trapping construct, and a vector containing such a gene-trapping construct, as well as a kit for the identification and/or isolation of genes, especially transient genes. The invention thus introduces a process for the identification and/or isolation of genes, particularly transient genes, and such a process is useful in identifying particular genes of interest
Abstract:The invention under consideration concerns a gene-trapping construct containing a first reporter gene which after activation can activate a second reporter gene, and the use of this gene-trapping construct for identification and isolation of genes, especially genes transiently expressed. The invention under consideration furthermore concerns a cell, preferably a mammalian cell, containing the abovementioned gene-trapping construct. The invention under consideration in addition concerns the use of this mammalian cell for identification and/or isolation of genes, particularly transient genes. Furthermore the invention concerns a vector containing the abovementioned gene-trapping construct, as well as a kit for identification and/or isolation of genes, especially transient genes, that contains at least the abovementioned gene-trapping construct or the abovementioned vector.In conclusion, the invention under consideration concerns a process for identification and/or isolation of genes, particularly transient genes.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6303327
Application Number:US1999000319495
Date Filed:08/09/1999
Date Published:16/10/2001
Assignee:Von Melchner; Harald, Frankfurt, Germany Holzer; Dieter, Frankfurt, Germany


 
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