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Stem Cell Related Patent Number US6306575

Title:Methods for preparing enriched human hematopoietic cell preparations
Inventors:Thomas, Terry; Vancouver, Canada
Lansdorp, Peter; Vancouver, Canada
Summary:Described herein are antibody compositions containing antibodies specific for glycophorin A, CD3, CD24, CD16, CD14, and optionally CD45RA, CD38, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and for antibodies specific for antigens expressed on non-hematopoietic tumor cells. Further disclosed is a negative selection process by which human hematopoietic progenitor cells and stem cells are enriched and recovered in a sample containing human hematopoietic differentiated progenitor cells, stem cells, and optionally tumor cells. Claims of the invention include processes of contacting and removal of the cell conjugates from which a cell preparation is obtained such that the human hematopoietic progenitor and stem cells contained therein have been enriched. Methods, materials and kits of the process are provided. Therapeutic applications include the depletion of metastatic tumor cells.
Abstract:The present invention relates to an antibody composition which contains antibodies specific for glycophorin A, CD3, CD24, CD16, CD14, and optionally CD45RA, CD38, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigens expressed on non-hematopoietic tumor cells. A process is also provided for enriching and recovering human hematopoietic progenitor cells and stem cells in a sample containing human hematopoietic differentiated, progenitor, and stem cells, and optionally tumor cells. The process involves reacting the sample with an antibody composition containing antibodies capable of binding to the antigens glycophorin A, CD3 CD24, CD16, and CD14, and optionally CD45RA, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigen expressed on non-hematopoietic tumor cells under conditions so that cell conjugates are formed between the antibodies and differentiated cells having the antigens glycophorin A, CD3 CD24, CD16, and CD14, and optionally CD45RA, CD38, CD36, CD38, CD56, CD2, CD19, CD66e, CD66b, and/or antibodies specific for antigens expressed on non-hematopoietic tumor cells on their surfaces. The cell conjugates are removed and a cell preparation is obtained which is enriched in human hematopoietic progenitor cells and stem cells. The invention also relates to kits for carrying out this process and to the cell preparations prepared by the process.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6306575
Application Number:US1995000566295
Date Filed:01/12/1995
Date Published:23/10/2001
Assignee:StemCell Technologies, Inc., Vancouver, Canada


 
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