Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6322784

Title:Adipogenic differentiation of human mesenchymal stem cells
Inventors:Pittenger, Mark F.; Severna Park, MD, USA
Beck, Stephen C.; Reistertown, MD, USA
Summary:Described herein are methods of inducing human mesenchymal stem cells (hMSCs) to differentiate into adipocytes, via a process of contacting the cells with defined inducer compounds. The invention relates to a composition comprised of hMSCs which have the potential to differentiate into cells of more than one connective tissue type, and a composition which induces cells from the hMSC population to differentiate into the adipogenic lineage. Claims of the invention include components of the composition for inducing such differentiation which include a glucocorticoid, a compound which stimulates cAMP production or inhibits cAMP degradation such as a phosphodiesterase inhibitor, and a compound which upregulates peroxisome proliferator activated receptor ? (PPAR ?) expression while increasing its binding affinity to the DNA binding site. Further disclosed are methods for isolating the adipocytes from the remaining hMSCs. Applications in surgical research are included.
Abstract:A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage, and a process for inducing such differentiation. The composition for inducing such differentiation comprises a glucocorticoid, a compound which stimulates cAMP production or inhibits cAMP degradation (such as a phosphodiesterase inhibitor), and/or a compound which upregulates peroxisome proliferator activated receptor .gamma. (PPAR .gamma.) expression and/or increases its binding affinity to its DNA binding site. The process can further include isolating the adipocytes from remaining hMSCs.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6322784
Application Number:US1998000246003
Date Filed:26/10/1998
Date Published:27/11/2001
Assignee:Osiris Therapeutics, Inc., Baltimore, MD, USA


 
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