Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6326198

Title:Methods and compositions for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
Inventors:Emerson, Stephen G.; Ann Arbor, MI, USA
Clarke, Michael F.; Ann Arbor, MI, USA
Palsson, Bernhard O.; Ann Arbor, MI, USA
Summary:Described herein are methods of culturing human stem cells via the use of a high culture medium exchange rate with the removal of metabolic products and the replenishing of depleted nutrients. The invention relates to methods and materials for the ex vivo human stem cell division and optimization of human hematopoietic progenitor cell cultures via an increase in the metabolism such as the GM-CSF secretion or IL-6 secretion of human stromal cells. Further disclosed are methods by which human stem cells or human hematopoietic progenitor cells or human stromal cells are cultured in a liquid medium which is preferably perfused either continuously or periodically at a rate of 1 ml of medium per ml of culture per approximately every 24 to about 48 hour period, after which metabolic products are removed and depleted nutrients are replenished while the culture is maintained under physiologically acceptable conditions and in the presence of growth factors.
Abstract:Methods, including culture media conditions, which provide for ex vivo human stem cell division and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6326198
Application Number:US1997000797730
Date Filed:11/02/1997
Date Published:04/12/2001
Assignee:Regents of the University of Michigan, Ann Arbor, MI, USA


 
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