Methods comprising apoptosis inhibitors for the generation of transgenic pigs

Stem Cell Clinic
Patient Application
FAQ
Contact
Locations
News
Videos
Research
Patents
3D Culture/Scaffold
Administration
Differentiation
Expansion
Extraction/Preservation
Mobilization
Type
USE
 


Stem Cell Related Patent Number US6331406

Title:Human enbryonic germ cell and methods of use
Inventors:Gearhart, John D.; Baltimore, MD, USA
Shamblott, Michael Joseph; Baltimore, MD, USA
Summary:This invention introduces novel cells with characteristics of human pluripotent embryonic germ cells, methods and uses thereof. Described herein are primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a human embryo 8 to 11 weeks old, after which the primordial germ cells are subject to long-term culturing of more than 30 days which results in cells that resemble embryonic stem cells in morphology and pluripotency. Further disclosed are methods by which the cells can be genetically manipulated using transgenic technology for the insertion of heterologous genetic material after the cells have been maintained in such a culture for several months. Therapeutic applications include screening for specific modulators, drugs and toxins and for generating cells of selected lineages.
Abstract:Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6331406
Application Number:US1997000989744
Date Filed:12/12/1997
Date Published:18/12/2001
Assignee:The John Hopkins University School of Medicine, Baltimore, MD, USA


 
Copyright © 2007 The Institute for Cellular Medicine  6/18/2021