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Stem Cell Related Patent Number US6333192

Title:Method of producing an undifferentiated avian cell culture using avian primordial germ cells
Inventors:Petitte, James N.; Raleigh, NC, USA
Chang, Il-Kuk; Raleigh, NC, USA
Summary:This invention describes the production of a sustained culture of undifferentiated avian cells expressing an embryonic stem cell phenotype, methods and uses thereof. Described herein is a process for the culturing of avian gonadal cells by which undifferentiated avian cells are produced which express an embryonic stem cell phenotype. Further disclosed are the means by which primordial germ cells are collected from an avian embryo after the formation of the primitive streak, at which point the avian gonadal cells are contacted with a preconditioned feeder matrix on which the avian gonadal cells are grown in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype. Applications are included for the production of transgenic avians such as chickens which express therapeutic proteins.
Abstract:A method of producing undifferentiated avian cells expressing an embryonic stem cell phenotype. The method includes the steps of collecting avian gonadal cells comprising primordial germ cells from an avian embryo after the formation of the primitive streak; depositing the avian gonadal cells in contact with a preconditioned feeder matrix; and growing the avian gonadal cells on the pre-conditioned feeder matrix in the presence of media for a time sufficient to produce an avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6333192
Application Number:US1999000370708
Date Filed:09/08/1999
Date Published:25/12/2001
Assignee:North Carolina State University, Raleigh, NC, USA


 
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