| Title: | Yeast artificial chromosomes and their use in the control of gene expression |
| Inventors: | Bruggemann, Marianne; Foxton, United Kingdom |
| Summary: | This invention describes the use of large DNA molecules in non-human animals via embryonic stem cells and yeast artificial chromosomes containing foreign DNA. The invention relates to murine embryonic stem cells or other cells that are essentially free of yeast DNA and which are prepared from suitably marked yeast artificial chromosomes and used to transfer large DNA segments into organisms. Further disclosed are methods by which such stem cells are transformed with a foreign gene or gene locus of at least 100 kb wherein the cell is essentially free of yeast DNA. |
| Abstract: | Embryonic stem cells or other cells (not prokaryotic or yeast) that are essentially free of yeast DNA are prepared from suitably marked yeast artificial chromosomes and used to transfer DNA segments of considerable size into organisms. |
| US Patent Website: | Click Here for Full Text of Patent |
| Title Number: | US6348349 |
| Application Number: | US1998000110811 |
| Date Filed: | 06/07/1998 |
| Date Published: | 19/02/2002 |
| Assignee: | The Babraham Institute United Kingdom |
