Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6355790

Title:Inhibition of HIV replication using a mutated transfer RNA primer
Inventors:Rosenblatt, Joseph D.; Rochester, NY, USA
Li, Xinqiang; San Diego, CA, USA
Summary:Described herein is a modified human tRNALys3 molecule for inhibiting HIV-1 replication. The invention relates to a novel modified transfer RNA for lysine that inhibits replication of the human immune deficiency virus and which exhibits reduced complementarity with a viral primer binding site. The modified tRNA molecules are described as having a modified aminoacyl acceptor stem with a 3' segment which has reduced complementarity to the HIV primer binding site and which can initiate aberrant reverse transcription. Additional properties of the tRNALys3 molecule include its modification in regions outside the acceptor stem which allows for interaction with reverse trancriptase or the HIV-1 RNA template and which can also inhibit HIV-1 replication. Therapeutic applications are included, such as in the introduction of the modified molecule into human cells for the inhibition of HIV-1 replication in the treatment of acquired immune deficiency syndrome and asymptomatic infections.
Abstract:Modified tRNA molecules are provided which inhibit HIV-1 replication. A tRNA molecule has a modified aminoacyl acceptor stem with a 3' segment which has reduced complementarity to the HIV primer binding site can initiate aberrant reverse transcription and/or interfere with reverse transcription. tRNA molecules which are modified in regions outside the acceptor stem which interact with reverse trancriptase or the HIV-1 RNA template can also inhibit HIV-1 replication. Methods are disclosed for introducing the modified tRNA molecules into human cells.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6355790
Application Number:US1997000866116
Date Filed:03/06/1997
Date Published:12/03/2002
Assignee:University of Rochester, Rochester, NY, USA University of California, Oakland, CA, USA


 
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