Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6369294

Title:Methods comprising apoptosis inhibitors for the generation of transgenic pigs
Inventors:Piedrahita, Jorge A.; College Station, TX
Bazer, Fuller W.; College Station, TX
Summary:This invention describes an improved method of growing porcine primordial germ cells from a porcine embryo. The cells are maintained on a matrix in which the high density of the feeder cells in the culture medium, in combination with a fibroblast growth factor and an apoptosis inhibitor, yield a high rate of efficiency at which the homologous recombination and transgenic production are obtained.
Abstract:Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6369294
Application Number:US2001000819964
Date Filed:28/03/2001
Date Published:09/04/2002
Assignee:Texas A and M University System, College Station, TX, USA


 
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