Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6372796

Title:Therapeutic uses for nitric oxide inhibitors
Inventors:Enikolopov, Grigori N.; Cold Spring Harbor, NY, USA
Peunova, Natalia I.; Cold Spring Harbor, NY, USA
Kuzin, Boris A.; Moscow, Russian Federation
Cline, Hollis; Cold Spring Harbor, NY, USA
Michurina, Tatyana; Moscow, Russian Federation
Summary:Described herein are methods of using inhibitors of nitric oxide or nitric oxide synthase to increase populations of cells in the S phase in tissue. Central to the invention is the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism, and described herein are methods of increasing a mammalian population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation in hematopoietic tissue, via contacting with NO. Further disclosed are methods by which the hematopoietic tissue is contacted with at least one inhibitor of NO, for purposes of tissue regenerating. Therapeutic applications in the regeneration of selected mammalian tissue are included.
Abstract:The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation in hematopoietic tissue, wherein the hematopoietic tissue is contacted with at least one inhibitor of NO, such as one or more inhibitors of nitric oxide synthase (NOS), thereby producing hematopoietic tissue having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis, differentiation and maturation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor (one or more) of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g., blood, skin, bone and digestive epithelium), or precursor cells of the selected tissue, with an inhibitor (one or more) of NO, thereby inhibiting differentiation and inducing proliferation of cells of the tissue.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6372796
Application Number:US1999000315929
Date Filed:20/05/1999
Date Published:16/04/2002
Assignee:Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA


 
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