Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6398816

Title:Genetic engineering of cells to enhance healing and tissue regeneration
Inventors:Breitbart, Arnold S.; Great Neck, NY, USA
Grande, Daniel A.; Sea Cliff, NY, USA
Mason, James M.; Bethpage, NY, USA
Summary:Described herein are methods of modified wound healing, cell proliferation and differentiation. The invention relates to a method for enhancing or increasing the efficiency of tissue repair, primarily of bone or cartilage, using genetically engineered cells. Further disclosed are methods by which mesenchymal stem cells are isolated from periosteum tissue, and transfected with the gene encoding a growth factor for the particular cell type to be repaired, whether by viral vectors, chemical transfectants, or by physico-mechanical methods such as electroporation and direct diffusion of DNA. Further therapeutic applications of such bioactive molecules are included, such as in the enhancement of the temporal sequence of wound repair, in the modification of the rate of cell proliferation, in the increase of metabolic synthesis of extracellular matrix proteins, and in the direct phenotypic expression in endogenous cell populations other than genetically engineered cells.
Abstract:A method for enhancing and/or increasing the efficiency of repair of tissues, primarily bone or cartilage, using genetically engineered cells has been developed. In the preferred embodiment, mesenchymal stem cells are isolated from periosteum tissue, and transfected with the gene encoding a growth factor for the particular cell type to be repaired. For example, for repair of bone, a gene (or genes) encoding bone morphogenic protein is transfected into periosteal cells. The transfected periosteal cells then express the bone morphogenic protein in culture to promote bone repair as a function of the expressed bone morphogenic protein. Cells can be transfected using any appropriate means, including viral vectors, as shown by the example, chemical transfectants, or physico-mechanical methods such as electroporation and direct diffusion of DNA. Genes can encode any useful protein, for example, a specific growth factor, morphogenesis factor, a structural protein, or a cytokine which enhances the temporal sequence of wound repair, alters the rate of proliferation, increases the metabolic synthesis of extracellular matrix proteins, or directs phenotypic expression in endogenous cell populations. Representative genes encoding proteins include bone growth factor genes, cartilage growth factor genes, nerve growth factor genes, and general growth factors important in wound healing, such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1), epidermal growth factor (EGF), basic fibroblast growth factor (FGF), endothelial derived growth supplement.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6398816
Application Number:US2000000489756
Date Filed:21/01/2000
Date Published:04/06/2002
Assignee:North Shore-Long Island Jewish Research Institute, Manhasset, NY, USA


 
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