Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6399369

Title:Multipotent neural stem cell cDNA libraries
Inventors:Weiss, Samuel; Calgary, Canada
Reynolds, Brent; Saltspring, Canada
Summary:Described herein are methods of obtaining a cDNA library from the proliferation of multipotent neural stem cells in a medium containing a growth factor for forming neurospheres. Further disclosed are methods by which the cDNA libraries may be obtained from both cultured normal and dysfunctional neural cells and from neural cell cultures at various stages of development. Claims of the invention include methods for the identification of the sequence of gene expression during neural development and applications in revealing the effects of biological agents on gene expression in neural cells. Methods are included for comparing nucleic acid derived from dysfunctional tissue with that of normal tissue to identify genetic material which may be a cause of the dysfunction. Additional therapeutic applications include the use of such cDNA libraries in the design of therapies to treat the neurological disorders, and in the diagnosis of genetic disorders or for use in identifying neural cells at various stages of development.
Abstract:cDNA libraries may be obtained from neural cell cultures produced by using growth factors to induce the proliferation of multipotent neural stem cells. The libraries may be obtained from both cultured normal and dysfunctional neural cells and from neural cell cultures at various stages of development. This information allows for the identification of the sequence of gene expression during neural development and can be used to reveal the effects of biological agents on gene expression in neural cells. Additionally, nucleic acid derived from dysfunctional tissue can be compared with that of normal tissue to identify genetic material which may be a cause of the dysfunction. This information could then be used in the design of therapies to treat the neurological disorder. A further use of the technology would be in the diagnosis of genetic disorders or for use in identifying neural cells at a particular stage in development.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6399369
Application Number:US1995000484203
Date Filed:07/06/1995
Date Published:04/06/2002
Assignee:NeuroSpheres Holdings Ltd., Calgary, Canada


 
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