Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6410320

Title:Method and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules
Inventors:Humes, H. David; Ann Arbor, MI, USA
Summary:This invention describes renal stem cells produced by culturing renal proximal tubule cells with a transforming growth factor-beta1, an epidermal growth factor and all-trans retinoic acid. Methods and compositions disclosed herein include culture media conditions which provide for the isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis. Further provided are methods of ex vivo expansion such that cells have been passaged at least three times, so that the population retains renal stem cells after at least three passages, and wherein the stem cells are capable of differentiating into renal proximal tubule cells. Therapeutic applications in the treatment of kidney failure are included.
Abstract:Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-.beta..sub.1, epidermal growth factor, and all-trans retinoic acid.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6410320
Application Number:US2000000494044
Date Filed:31/01/2000
Date Published:25/06/2002
Assignee:The University of Michigan, Ann Arbor, MI, USA


 
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