Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6423829

Title:Nitrobenzylmercaptopurineriboside (NBMPR)-insensitive, equilibrative, nucleoside transport protein, nucleic acids encoding the same and methods of use
Inventors:Belt, Judith A.; Memphis, TN, USA
Crawford, Charles R.; Memphis, TN, USA
Patel, Divyen H.; Memphis, TN, USA
Summary:Described herein are a novel nitrobenzylmercaptopurineriboside (NBMPR)-insensitive, equilibrative nucleoside transport protein (iENTP) and nucleic acid. The invention relates to an isolated NBMPR-iENTP and the nucleic acid encoding it, such that the iENTP can be used in screening assays to identify both natural nucleoside perrneants as well as inhibitors and analogs thereof. Further disclosed are transfected or transduced cell lines which use the iENTP as the sole nucleoside transport protein. Applications in drug screening and in a chemotherapy protocol are included, as are methods of using these cells to selectively express a heterologous gene for gene therapy.
Abstract:An isolated NBMPR-insensitive equilibrative nucleoside transport protein (iENTP) and the nucleic acid encoding it is disclosed. The iENTP can be used in screening assays to identify both natural nucleoside perrneants and/or inhibitors and analogs thereof. In addition, transfected or transduced cell lines are disclosed which use the iENTP as the sole nucleoside transport protein. Methods of employing such cell lines for drug screening are also included. Furthermore methods of using hematopoietic stem cells transduced with an iENTP in a chemotherapy protocol is also described. In addition, methods of using these cells to selectively express a heterologous gene for gene therapy is disclosed.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6423829
Application Number:US2000000611781
Date Filed:07/07/2000
Date Published:23/07/2002
Assignee:St. Jude Children's Research Hospital, Memphis, TN, USA


 
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