Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6440734

Title:Methods and devices for the long-term culture of hematopoietic progenitor cells
Inventors:Pykett, Mark J.; Newton, MA, USA
Rosenzweig, Michael; Boston, MA, USA
Kaplan, Richard B.; Beverly Hills, CA, USA
Summary:This invention introduces a novel process for the long term in vitro culturing of hematopoietic progenitor cells. Described herein are methods and devices for the culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, with improved methods for the introduction of foreign genetic material into cells of hematopoietic origin. Further disclosed are related apparatus materials associated with such methods. Claims of the invention include a three-dimensional porous biomaterial on which the hematopoietic progenitor cells are cultured, such that the three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. Also provided are the means by which this three-dimensional porous biomaterial supports high level transduction on cells cultured in such environment.
Abstract:The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6440734
Application Number:US2000000509379
Date Filed:07/06/2000
Date Published:27/08/2002
Assignee:Cytomatrix, LLC, Woburn, MA, USA


 
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