Methods comprising apoptosis inhibitors for the generation of transgenic pigs

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Stem Cell Related Patent Number US6465247

Title:Mammalian myeloid progenitor cell subsets
Inventors:Weissman, Irving L.; Redwood City, CA, USA
Traver, David Jeffrey; West Roxbury, MA, USA
Akashi, Koichi; Palo Alto, CA, USA
Summary:Described herein is a mammalian myeloid progenitor cell composition comprised of a substantially enriched mammalian hematopoietic cell subpopulation, which is characterized by progenitor cell activity for myeloid lineages, but which lacks the potential to differentiate into lymphoid lineages. Claims of the invention include further dividing the population into specific myeloid progenitor subsets, including common myeloid progenitor cells (CMP), megakaryocyte/ erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Further disclosed are methods for the isolation and culture of these subpopulations, such that the CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/ megakaryocytic or myelomonocytic lineages. Through the cell enrichment methods the cells have been shown to give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo. Applications in therapeutic transplantation and in the experimental evaluation of a source of lineage- and cell-specific products are included.
Abstract:A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for myeloid lineages, but lacking the potential to differentiate into lymphoid lineages. This population is further divided into specific myeloid progenitor subsets, including a common myeloid progenitor cells (CMP), megakaryocyte/erythroid progenitor cells (MEP) and granulocyte/monocyte lineage progenitor (GMP). Methods are provided for the isolation and culture of these subpopulations. The CMP population gives rise to all myeloid lineages, and can give rise to the two additional and isolatable progenitor populations that are exclusively committed to either the erythroid/megakaryocytic or myelomonocytic lineages. The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor .alpha.); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. These cells give rise to a variety of myeloid cells, including megakaryocytes, granulocytes, dendritic cells and erythroid cells, as evidenced by their growth and differentiation in vitro and in vivo.
US Patent Website:Click Here for Full Text of Patent
Title Number:US6465247
Application Number:US2000000607529
Date Filed:29/06/2000
Date Published:15/10/2002
Assignee:The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA, USA


 
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